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Archive for the ‘Documentation’ Category

BioRuby paper published

Thursday, August 26th, 2010

After 10 years of development, the BioRuby paper is finally published in the Bioinformatics journal.  The article is open access, so please take a look. BioRuby: Bioinformatics software for the Ruby programming language Naohisa Goto, Pjotr Prins, Mitsuteru Nakao, Raoul Bonnal, Jan Aerts and Toshiaki Katayama Bioinformatics 2010; doi: 10.1093/bioinformatics/btq475

BioPerl has moved to GitHub

Friday, May 14th, 2010

BioPerl has migrated to git and GitHub!  We have also set up a mirror set of several key repositories at the great public git hosting site repo.or.cz. If you are a current BioPerl developer (had a previous account for direct access to our prior Subversion repository), please sign up for a GitHub ...

Illumina FASTQ files – Read Segment Quality Control Indicator

Friday, April 30th, 2010

In another quirk to the FASTQ story, recent Illumina FASTQ files don't actually use the full range of PHRED scores - and a score of 2 has a special meaning, The Read Segment Quality Control Indicator (RSQCI, encoded as 'B'). Hats off to Dr Torsten Seemann for raising awareness of this ...

Making Biopython SeqIO and AlignIO easier

Monday, April 5th, 2010

One of the small changes coming in Biopython 1.54 (which you can try out already using the Biopython 1.54 beta) is to Bio.SeqIO and Bio.AlignIO. Previously the input and output functions had required file handles, but they will now also accept filenames. This is a case of practicality beats purity ...

Sanger FASTQ format and the Solexa/Illumina variants

Thursday, December 17th, 2009

I'm delighted to announce an open access publication in Nucleic Acids Research describing the FASTQ file format based on the conventions agreed by the OBF projects: The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants Peter J. A. Cock (Biopython), Christopher J. Fields (BioPerl), Naohisa Goto ...

Interleaving paired FASTQ files with Biopython

Monday, December 14th, 2009

This post is about paired end data (FASTA or FASTQ) and manipulating it with Biopython's Bio.SeqIO module (see also FASTQ conversions & speeding up FASTQ).

BioPerl core 1.6.1 PPM available

Saturday, October 10th, 2009

BioPerl 1.6.1 is now available for ActivePerl as a PPM, instructions for downloading can be found on the BioPerl wiki. This has been tested only for ActivePerl 5.10 and above, so any feedback with older versions of BioPerl would be greatly appreciated.

BioPerl 1.6.1 released

Tuesday, September 29th, 2009

We are pleased to announce the immediate availability of BioPerl 1.6.1, the latest release of BioPerl's core code. You can grab it here: Via CPAN: http://search.cpan.org/~cjfields/BioPerl-1.6.1/ Via the BioPerl website: http://bioperl.org/DIST/BioPerl-1.6.1.tar.bz2 http://bioperl.org/DIST/BioPerl-1.6.1.tar.gz http://bioperl.org/DIST/BioPerl-1.6.1.zip The PPM for Windows should also finally be available this week, ActivePerl problems permitting (we will post more information when it ...

Working with FASTQ files in Biopython when speed matters

Friday, September 25th, 2009

Biopython 1.51 onward includes support for Sanger, Solexa and Illumina 1.3+ FASTQ files in Bio.SeqIO, which allows a lot of neat tricks very concisely. For example, the tutorial (PDF) has examples finding and removing primer or adaptor sequences. However, because the Bio.SeqIO interface revolves around SeqRecord objects there is often a ...

Biopython CVS to git migration

Thursday, September 24th, 2009

The release of Biopython 1.52 earlier this week marked the end of an era, it was our last release using CVS for source code control. As of now, Biopython is using a git repository, hosted on github.com who kindly provide git hosting for open source projects free of charge. The BioRuby ...